中文 
别名:GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras, ERAS, HRAS2, HRASP应用:WB,IHC
反应种属:Human
规格：50μl/100μl
| Description |
|---|
| Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells. |
| Specification | |
|---|---|
| Aliases | GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras, ERAS, HRAS2, HRASP |
| Entrez GeneID | 3266 |
| Swissprot | Q7Z444 |
| WB Predicted band size | 25.3kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20~50 amino acids from the N-terminal region of human ERAS. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Application | |
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
 |
Anti-ERAS Antibody (N-term) (A28) at 1:1000 dilution + Hela whole cell lysate
Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
 |
Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS antibody (N-term) (A28), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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