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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to ERVK-7 (N-Term)

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别名:Endogenous retrovirus group K member 7 Env polyprotein, Envelope polyprotein, HERV-K(III) envelope protein, HERV-K102 envelope protein, HERV-K_1q22 provirus ancestral Env polyprotein, Surface protein, SU, Transmembrane protein, TM, ERVK-7
应用:WB,IHC
反应种属:Human
规格:50μl/100μl

Description
Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. TM anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane (By similarity).
Specification
Aliases Endogenous retrovirus group K member 7 Env polyprotein, Envelope polyprotein, HERV-K(III) envelope protein, HERV-K102 envelope protein, HERV-K_1q22 provirus ancestral Env polyprotein, Surface protein, SU, Transmembrane protein, TM, ERVK-7
Swissprot P61567
WB Predicted band size 66.6kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This ERVK-7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 96-128 amino acids from human ERVK-7.
Application
WB 1/1000-1/2000
IHC 1/100-1/500

All lanes : Anti-ERVK-7 Antibody (N-Term) at 1:1000-1:2000 dilution
Lane 1: Raji whole cell lysate
Lane 2: human lung lysate
Lane 3: human testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 67 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

P33477 staining ERVK-7 in human placenta tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。