Rabbit Polyclonal Antibody to ESPL1

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别名:ESP1; SEPA
应用:WB,IHC
反应种属:Human, Mouse
规格：50μl/100μl

Description
Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).

Description

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).

Specification
Aliases	ESP1; SEPA
Swissprot	Q14674
WB Predicted band size	233 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	Synthetic peptide of human ESPL1
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/200-1/1000
IHC	1/25-1/100
ELISA	1/1000-1/2000

	Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Raji cells, Primary antibody: P04858(ESPL1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second
	The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P04858(ESPL1 Antibody) at dilution 1/40. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P04858(ESPL1 Antibody) at dilution 1/40. (Original magnification: ×200)