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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to ESPN (N-term)

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别名:Espin, Autosomal recessive deafness type 36 protein, Ectoplasmic specialization protein, ESPN, DFNB36
应用:WB,IHC,FCM
反应种属:Human
规格:50μl/100μl

Description
ESPN is a multifunctional actin-bundling protein. It plays a major role in regulating the organization, dimensions, dynamics, and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in various mechanosensory and chemosensory cells.
Specification
Aliases Espin, Autosomal recessive deafness type 36 protein, Ectoplasmic specialization protein, ESPN, DFNB36
Entrez GeneID 83715
Swissprot B1AK53
WB Predicted band size 91.7kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This ESPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-45 amino acids from the N-terminal region of human ESPN.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
IHC 1/100-1/500
FCM 1/10-1/50

Anti-ESPN Antibody (N-term) at 1:1000 dilution + SK-BR-3 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 92 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of ESPN Antibody (N-term) (Cat. #P33653) in 293 cell line lysates (35ug/lane). ESPN (arrow) was detected using the purified Pab.
ESPN Antibody (N-term) (Cat. #P33653) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human testis tissue reacted with ESPN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。