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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to ETFA

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别名:Electron transfer flavoprotein subunit alpha, mitochondrial, Alpha-ETF, ETFA
应用:WB,ICC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl- CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
Specification
Aliases Electron transfer flavoprotein subunit alpha, mitochondrial, Alpha-ETF, ETFA
Entrez GeneID 2108
Swissprot P13804
WB Predicted band size 35.1kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This ETFA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 192-226 amino acids from the Central region of human ETFA.
Application
WB 1/1000
ICC 1/25

Western blot analysis of lysates from A431, Hela, HepG2 cell line , human liver and mouse liver tissue lysate(from left to right), using ETFA Antibody (Center)(Cat. #P32649). P32649 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Fluorescent image of Hela cells stained with ETFA Antibody (Center)(Cat#P32649). P32649 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。