Rabbit Polyclonal Antibody to ETFA

包装: 1G|100G|500G|1KG|100KG

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别名:Electron transfer flavoprotein subunit alpha, mitochondrial, Alpha-ETF, ETFA
应用:WB,IHC,FCM
反应种属:Human, Mouse
规格：50μl/100μl

Description
ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.

Description

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.

Specification
Aliases	Electron transfer flavoprotein subunit alpha, mitochondrial, Alpha-ETF, ETFA
Entrez GeneID	2108
Swissprot	P13804
WB Predicted band size	35.1kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	This ETFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the C-terminal region of human ETFA.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	ETFA Antibody (C-term) (Cat. #P30922) western blot analysis in K562 cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the ETFA antibody detected the ETFA protein (arrow).
	ETFA Antibody (C-term) (Cat. #P30922) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	ETFA Antibody (C-term) (Cat. #P30922)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ETFA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.