中文 
别名:Fatty acid desaturase 2, 11419-, Delta(6) fatty acid desaturase, D6D, Delta(6) desaturase, Delta-6 desaturase, FADS2应用:WB,FCM
反应种属:Human
规格：50μl/100μl
| Description |
|---|
| The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. |
| Specification | |
|---|---|
| Aliases | Fatty acid desaturase 2, 11419-, Delta(6) fatty acid desaturase, D6D, Delta(6) desaturase, Delta-6 desaturase, FADS2 |
| Entrez GeneID | 9415 |
| Swissprot | O95864 |
| WB Predicted band size | 52.3kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This FADS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-108 amino acids from the N-terminal region of human FADS2. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Application | |
|---|---|
| WB | 1/1000 |
| FCM | 1/25 |
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All lanes : Anti-FADS2 Antibody (N-term) at 1:1000 dilution Lane 1: Human breast lysate Lane 2:Human liver lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 60 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Overlay histogram showing HepG2 cells stained with P34576(green line). The cells were fixed with 2% paraformaldehyde 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34576, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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