Rabbit Polyclonal Antibody to FADS2

包装: 1G|100G|500G|1KG|100KG

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别名:Fatty acid desaturase 2, 11419-, Delta(6) fatty acid desaturase, D6D, Delta(6) desaturase, Delta-6 desaturase, FADS2
应用:WB,IHC,FCM
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization.

Description

The protein encoded by this gene is a member of the fatty
acid desaturase (FADS) gene family. Desaturase enzymes regulate
unsaturation of fatty acids through the introduction of double
bonds between defined carbons of the fatty acyl chain. FADS family
members are considered fusion products composed of an N-terminal
cytochrome b5-like domain and a C-terminal multiple
membrane-spanning desaturase portion, both of which are
characterized by conserved histidine motifs. This gene is clustered
with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is
thought to have arisen evolutionarily from gene duplication based
on its similar exon/intron organization.

Specification
Aliases	Fatty acid desaturase 2, 11419-, Delta(6) fatty acid desaturase, D6D, Delta(6) desaturase, Delta-6 desaturase, FADS2
Entrez GeneID	9415
Swissprot	O95864
WB Predicted band size	52.3kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This FADS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-122 amino acids of human FADS2.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	FADS2 Antibody (Center) (Cat. #P30673) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FADS2 antibody detected the FADS2 protein (arrow).
	FADS2 Antibody (Center) (Cat. #P30673) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Immunohistochemical analysis of paraffin-embedded H.brain section using FADS2 Antibody (Center)(Cat#P30673). P30673 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.