中文 
别名:Multivesicular body subunit 12A, CIN85/CD2AP family-binding protein, ESCRT-I complex subunit MVB12A, Protein FAM125A, MVB12A, CFBP, FAM125A应用:WB,ICC,FCM
反应种属:Human
规格：50μl/100μl
| Description |
|---|
| Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in the ligand-mediated internalization and down- regulation of EGF receptor. |
| Specification | |
|---|---|
| Aliases | Multivesicular body subunit 12A, CIN85/CD2AP family-binding protein, ESCRT-I complex subunit MVB12A, Protein FAM125A, MVB12A, CFBP, FAM125A |
| Entrez GeneID | 93343 |
| Swissprot | Q96EY5 |
| WB Predicted band size | 28.8kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This FAM125A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-50 amino acids from human FAM125A. |
| Application | |
|---|---|
| WB | 1/2000 |
| ICC | 1/25 |
| FCM | 1/25 |
 |
Anti-FAM125A Antibody (N-Term) at 1:2000 dilution + PC-3 whole cell lysate
Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
 |
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling FAM125A with P34434 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue). |
 |
Overlay histogram showing A431 cells stained with P34434(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
暂无评论
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
发表回复