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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to Fascin-3 (N-term)

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别名:Fascin-3, Testis fascin, FSCN3
应用:WB,IHC,FCM
反应种属:Human, Mouse
规格:50μl/100μl

Description
FSCN3 acts as an actin bundling protein.
Specification
Aliases Fascin-3, Testis fascin, FSCN3
Entrez GeneID 29999
Swissprot Q9NQT6
WB Predicted band size 56.6kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This Fascin-3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Fascin-3.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/1000-1/8000
IHC 1/100-1/500
FCM 1/10-1/50

Anti-Fascin-3 Antibody (N-term) at 1:8000 dilution + human testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 57 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of Fascin-3 Antibody (N-term) (Cat. #P33857) in MDA-MB231, NCI-H460 cell line and mouse testis tissue lysates (35ug/lane). Fascin-3 (arrow) was detected using the purified Pab.
Fascin-3 Antibody (N-term) (Cat. #P33857) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Fascin-3 Antibody (N-term) (Cat. #P33857) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Fascin-3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。