Rabbit Polyclonal Antibody to FCN3

包装: 1G|100G|500G|1KG|100KG

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别名:Ficolin-3, Collagen/fibrinogen domain-containing lectin 3 p35, Collagen/fibrinogen domain-containing protein 3, Hakata antigen, FCN3, FCNH, HAKA1
应用:WB,IHC,FCM
反应种属:Human
规格：50μl/100μl

Description
Ficolins are a group of proteins which consist of a collagen-like domain and a fibrinogen-like domain. In human serum, there are two types of ficolins, both of which have lectin activity. The protein encoded by this gene is a thermolabile beta-2-macroglycoprotein found in all human serum and is a member of the ficolin/opsonin p35 lectin family. The protein, which was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus, has been shown to have a calcium-independent lectin activity. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Alternative splicing occurs at this locus and two variants, each encoding a distinct isoform, have been identified.

Description

Ficolins are a group of proteins which consist of a
collagen-like domain and a fibrinogen-like domain. In human serum,
there are two types of ficolins, both of which have lectin
activity. The protein encoded by this gene is a thermolabile
beta-2-macroglycoprotein found in all human serum and is a member
of the ficolin/opsonin p35 lectin family. The protein, which was
initially identified based on its reactivity with sera from
patients with systemic lupus erythematosus, has been shown to have
a calcium-independent lectin activity. The protein can activate the
complement pathway in association with MASPs and sMAP, thereby
aiding in host defense through the activation of the lectin
pathway. Alternative splicing occurs at this locus and two
variants, each encoding a distinct isoform, have been identified.

Specification
Aliases	Ficolin-3, Collagen/fibrinogen domain-containing lectin 3 p35, Collagen/fibrinogen domain-containing protein 3, Hakata antigen, FCN3, FCNH, HAKA1
Entrez GeneID	8547
Swissprot	O75636
WB Predicted band size	32.9kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This FCN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-243 amino acids from the C-terminal region of human FCN3.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	All lanes : Anti-FCN3 Antibody (C-term) at 1:1000 dilution Lane 1: HACAT whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 33kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	FCN3 Antibody (C-term) (Cat. #P34977) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the FCN3 antibody detected the FCN3 protein (arrow).
	FCN3 Antibody (C-term) (Cat. #P34977) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
	FCN3 Antibody (C-term) (Cat. #P34977)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FCN3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.