Rabbit Polyclonal Antibody to FEN1

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别名:Flap endonuclease 1; EC 3.1.-.-; Flap structure-specific endonuclease 1; FEN-1; Maturation factor 1
应用:WB,IHC,ICC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Structure-specific nuclease with 5′-flap endonuclease and 5′-3′ exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5′-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5′-end of a downstream Okazaki fragment. It enters the flap from the 5′-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5′-3′ exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.Murray J.M., Mol. Cell. Biol. 14:4878-4888(1994).Hiraoka L.R., Genomics 25:220-225(1995).Taylor T.D., Nature 440:497-500(2006).

Description

Structure-specific nuclease with 5′-flap endonuclease and 5′-3′ exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5′-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5′-end of a downstream Okazaki fragment. It enters the flap from the 5′-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5′-3′ exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.Murray J.M., Mol. Cell. Biol. 14:4878-4888(1994).Hiraoka L.R., Genomics 25:220-225(1995).Taylor T.D., Nature 440:497-500(2006).

Specification
Aliases	Flap endonuclease 1; EC 3.1.-.-; Flap structure-specific endonuclease 1; FEN-1; Maturation factor 1
Entrez GeneID	2237;
Swissprot	P39748
WB Predicted band size	42kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from internal of human FEN1.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100
ICC	1/100-1/500

	Western blot analysis of extracts from Jurkat cells, 293 cells and HUVEC cells, using FEN1 antibody P41944.
	Immunofluorescence analysis of HeLa cells, using FEN1 antibody P41944.
	Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using FEN1 antibody P41944.