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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to FEV (N-Term)

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别名:Protein FEV, Fifth Ewing variant protein, PC12 ETS domain-containing transcription factor 1, PC12 ETS factor 1, Pet-1, FEV, PET1
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
Functions as a transcriptional regulator. According to PubMed:12761502, it functions as a transcriptional repressor. Functions in the differentiation and the maintenance of the central serotonergic neurons. May play a role in cell growth.
Specification
Aliases Protein FEV, Fifth Ewing variant protein, PC12 ETS domain-containing transcription factor 1, PC12 ETS factor 1, Pet-1, FEV, PET1
Entrez GeneID 54738
Swissprot Q99581
WB Predicted band size 25.0kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This FEV antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 17-49 amino acids from human FEV.
Application
WB 1/2000
IHC 1/100-1/500

Anti-FEV Antibody (N-Term) at 1:2000 dilution + K562 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 25 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

P33322 staining FEV in human small intestine sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。