Rabbit Polyclonal Antibody to FGFR1 (Ab-766)

包装: 1G|100G|500G|1KG|100KG

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别名:EC 2.7.10.1; FGFBR; FGFR-1; FGR1; FLG
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.Isacchi A., Nucleic Acids Res. 18:1906-1906(1990).Popovici C., Blood 93:1381-1389(1999).Liu T., J. Proteome Res. 4:2070-2080(2005).

Description

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.Isacchi A., Nucleic Acids Res. 18:1906-1906(1990).Popovici C., Blood 93:1381-1389(1999).Liu T., J. Proteome Res. 4:2070-2080(2005).

Specification
Aliases	EC 2.7.10.1; FGFBR; FGFR-1; FGR1; FLG
Entrez GeneID	2260;
Swissprot	P11362
WB Predicted band size	120kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized non-phosphopeptide derived from human FGFR1 around the phosphorylation site of tyrosine 766 (Q-E-Y(p)-L-D).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from HepG2 cells, using FGFR1 (Ab-766) antibody P41645.
	Western blot analysis of extracts from HepG2 cells (Lane 2), using FGFR1 (Ab-766) antiobdy P41645. The lane on the left is treated with synthesized peptide.
	Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using FGFR1 (Ab-766) antiobdy P41645.