Rabbit Polyclonal Antibody to Fibrillarin

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别名:FIB; FLRN; Nop1; RNU3IP1
应用:WB,IHC,ICC,FCM,IP
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Fibrillarin is a 2′-O-methyltransferase located in fibrillar regions and Cajal bodies of the nucleolus, where RNA transcription and pre-RNA processing take place. Fibrillarin associates with several other structual proteins as well as box C/D snoRNA to form a complex that functions in pre-rRNA processing, pre-rRNA methylation and ribosome assembly. This complex catalyzes site-specific 2′-O-ribose methylation of targeted nucleotides within the rRNA sequence. The sequence, structure and function of fibrillarin are highly conserved and fibrillarin gene expression is essential for early embryonic development.

Description

Fibrillarin is a 2′-O-methyltransferase located in fibrillar regions and Cajal bodies of the nucleolus, where RNA transcription and pre-RNA processing take place. Fibrillarin associates with several other structual proteins as well as box C/D snoRNA to form a complex that functions in pre-rRNA processing, pre-rRNA methylation and ribosome assembly. This complex catalyzes site-specific 2′-O-ribose methylation of targeted nucleotides within the rRNA sequence. The sequence, structure and function of fibrillarin are highly conserved and fibrillarin gene expression is essential for early embryonic development.

Specification
Aliases	FIB; FLRN; Nop1; RNU3IP1
Entrez GeneID	2091
Swissprot	P22087
WB Predicted band size	Calculated MW: 34 kDa; Observed MW: 34 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	A synthesized peptide derived from human Fibrillarin
Formulation	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200
FCM	1/50-1/100

	Western blot analysis of Fibrillarin in HepG2 lysates using Fibrillarin antibody.
	Immunofluorescence analysis of Fibrillarin in Hela using Fibrillarin antibody.
	Immunohistochemistry analysis of paraffin-embedded Human testis using Fibrillarin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.