别名:DDDDK epitope tag; DYKDDDDK epitope tag应用:WB
反应种属:Fusion proteins containing the Flag Tag
规格:50μl/100μl
| Description |
|---|
| The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein’s biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins. |
| Specification | |
|---|---|
| Aliases | DDDDK epitope tag; DYKDDDDK epitope tag |
| WB Predicted band size | 50 kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Fusion proteins containing the Flag Tag |
| Immunogen | Synthetic peptide: DYKDDDDK conjugated to KLH |
| Formulation | pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
| Application | |
|---|---|
| WB | 1/2000-1/10000 |
| ELISA | 1/5000-1/10000 |
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Gel: 8%SDS-PAGE, Lysate: 0.5 μg, Lane: Fusion proteins containing the flag Tag, Primary antibody: P00005(FLAG Tag Antibody) at dilution 1/5000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 2 minutes |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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