Rabbit Polyclonal Antibody to GABA B Receptor

包装: 1G|100G|500G|1KG|100KG

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别名:GABABR2; GPR51; G protein-coupled receptor 51; GABA-B RECEPTOR; R2 SUBUNIT
应用:WB,IHC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Calcium is required for high affinity binding to GABA. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Activated by (-)-baclofen, cgp27492 and blocked by phaclofen. Isoform 1E may regulate the formation of functional GABBR1/GABBR2 heterodimers by competing for GABBR2 binding. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.Xinmin Xie, J. Physiol., Jul 2006; 574: 399 – 414.A. Gambardella, Neurology, Feb 2003; 60: 560 – 563.P Tosetti, J. Physiol., Nov 2005; 568: 951 – 966.Marta Margeta-Mitrovic, PNAS, Dec 2001; 98: 14643.

Description

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Calcium is required for high affinity binding to GABA. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Activated by (-)-baclofen, cgp27492 and blocked by phaclofen. Isoform 1E may regulate the formation of functional GABBR1/GABBR2 heterodimers by competing for GABBR2 binding. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.Xinmin Xie, J. Physiol., Jul 2006; 574: 399 – 414.A. Gambardella, Neurology, Feb 2003; 60: 560 – 563.P Tosetti, J. Physiol., Nov 2005; 568: 951 – 966.Marta Margeta-Mitrovic, PNAS, Dec 2001; 98: 14643.

Specification
Aliases	GABABR2; GPR51; G protein-coupled receptor 51; GABA-B RECEPTOR; R2 SUBUNIT
Entrez GeneID	2550;
Swissprot	Q9UBS5
WB Predicted band size	110kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human GABA B receptor.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from HeLa cells, using GABA B Receptor antibody P41801.
	Western blot analysis of extracts from JK cells (Lane 2) and A549 cells (Lane 3), using GABA-B Receptor antiobdy P41801. The lane on the left is treated with synthesized peptide.
	Immunohistochemical analysis of paraffin-embedded human brain tissue using GABA B Receptor antibody P41801.