Rabbit Polyclonal Antibody to GALNT4 (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:Polypeptide N-acetylgalactosaminyltransferase 4, Polypeptide GalNAc transferase 4, GalNAc-T4, pp-GaNTase 4, Protein-UDP acetylgalactosaminyltransferase 4, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4, GALNT4
应用:WB,FCM
反应种属:Human
规格：50μl/100μl

Description
This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. In vitro, the encoded protein can complement other GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem repeat and can O-glycosylate the P-selectin glycoprotein ligand-1 molecule. The coding region of this gene is contained within a single exon.

Description

This gene encodes a member of the
UDP-N-acetyl-alpha-D-galactosamine:polypeptide
N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes.
GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi
apparatus by catalyzing the transfer of GalNAc to serine and
threonine residues on target proteins. They are characterized by an
N-terminal transmembrane domain, a stem region, a lumenal catalytic
domain containing a GT1 motif and Gal/GalNAc transferase motif, and
a C-terminal ricin/lectin-like domain. GalNAc-Ts have different,
but overlapping, substrate specificities and patterns of
expression. In vitro, the encoded protein can complement other
GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem
repeat and can O-glycosylate the P-selectin glycoprotein ligand-1
molecule. The coding region of this gene is contained within a
single exon.

Specification
Aliases	Polypeptide N-acetylgalactosaminyltransferase 4, Polypeptide GalNAc transferase 4, GalNAc-T4, pp-GaNTase 4, Protein-UDP acetylgalactosaminyltransferase 4, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4, GALNT4
Entrez GeneID	100528030;8693
Swissprot	Q8N4A0
WB Predicted band size	66.7kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This GALNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-120 amino acids from the N-terminal region of human GALNT4.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
FCM	1/10-1/50

	GALNT4 Antibody (N-term) (Cat. #P30738) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the GALNT4 antibody detected the GALNT4 protein (arrow).
	GALNT4 Antibody (N-term) (Cat. #P30738) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.