中文 
别名:Tubulin gamma-2 chain, Gamma-2-tubulin, Tubg2应用:WB,FCM
反应种属:Human, Mouse
规格：50μl/100μl
| Description |
|---|
| Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation (By similarity). |
| Specification | |
|---|---|
| Aliases | Tubulin gamma-2 chain, Gamma-2-tubulin, Tubg2 |
| Entrez GeneID | 103768 |
| Swissprot | Q8VCK3 |
| WB Predicted band size | 51.1kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This gamma Tubulin antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 400-434 amino acids from the mouse region of mouse gamma Tubulin. |
| Application | |
|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
 |
All lanes : Anti-gamma Tubulin Antibody at 1:2000 dilution Lane 1: Mouse brain lysate Lane 2: Mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Overlay histogram showing C2C12 cells stained with P34527(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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