Rabbit Polyclonal Antibody to GCLC

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别名:GCL; GCS; GLCL; GLCLC
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

Description

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

Specification
Aliases	GCL; GCS; GLCL; GLCLC
Swissprot	P48506
WB Predicted band size	73 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	Fusion protein of human GCLC
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/500-1/2000
IHC	1/25-1/100
ELISA	1/5000-1/10000

	Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-4: Raw264.7, A549, Hepg2 and Jurkat cell, Primary antibody: P02233(GCLC Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
	The image is immunohistochemistry of paraffin-embedded Human brain tissue using P02233(GCLC Antibody) at dilution 1/35. (Original magnification: ×200)