库存-现货
瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to GDE1

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别名:Glycerophosphodiester phosphodiesterase 1, Membrane-interacting protein of RGS16, RGS16-interacting membrane protein, GDE1, MIR16
应用:WB,FCM
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
GDE1 has glycerophosphoinositol phosphodiesterase activity. Has little or no activity towards glycerophosphocholine. GDE1 activity can be modulated by G-protein signaling pathways (By similarity).
Specification
Aliases Glycerophosphodiester phosphodiesterase 1, Membrane-interacting protein of RGS16, RGS16-interacting membrane protein, GDE1, MIR16
Entrez GeneID 51573
Swissprot Q9NZC3
WB Predicted band size 37.7kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This GDE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-219 amino acids of human GDE1.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
FCM 1/25

Anti-GDE1 Antibody (Center) at 1:1000 dilution + Rat heart lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 38 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing Hela cells stained with P34499(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34499, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。