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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to GHSR

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别名:Growth hormone secretagogue receptor type 1, GHS-R, GH-releasing peptide receptor, GHRP, Ghrelin receptor, GHSR
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
This gene encodes a member of the G-protein coupled
receptor family. The encoded protein may play a role in energy
homeostasis and regulation of body weight. Two identified
transcript variants are expressed in several tissues and are
evolutionary conserved in fish and swine. One transcript, 1a,
excises an intron and encodes the functional protein; this protein
is the receptor for the Ghrelin ligand and defines a neuroendocrine
pathway for growth hormone release. The second transcript (1b)
retains the intron and does not function as a receptor for Ghrelin;
however, it may function to attenuate activity of isoform 1a.
Mutations in this gene are associated with autosomal idiopathic
short stature.
Specification
Aliases Growth hormone secretagogue receptor type 1, GHS-R, GH-releasing peptide receptor, GHRP, Ghrelin receptor, GHSR
Entrez GeneID 2693
Swissprot Q92847
WB Predicted band size 41.3kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This GHSR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-357 amino acids from the C-terminal region of human GHSR.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
IHC 1/100-1/500

All lanes :GHSR Antibody (C-term) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: mouse brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/10000 dilution.

Observed band size : 45kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

GHSR Antibody (C-term) (Cat. #P34903) western blot analysis in NCI-H292 cell line and mouse bladder tissue lysates (35ug/lane).This demonstrates the GHSR antibody detected the GHSR protein (arrow).
GHSR Antibody (C-term) (Cat. #P34903)immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GHSR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。