别名:Gastric intrinsic factor, Intrinsic factor, IF, INF, GIF, IFMH应用:WB,IHC,ICC,FCM
反应种属:Human, Mouse
规格:50μl/100μl
| Description |
|---|
| This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. |
| Specification | |
|---|---|
| Aliases | Gastric intrinsic factor, Intrinsic factor, IF, INF, GIF, IFMH |
| Entrez GeneID | 2694 |
| Swissprot | P27352 |
| WB Predicted band size | 45.4kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This GIF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-218 amino acids from the Central region of human GIF. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Application | |
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| ICC | 1/10-1/50 |
| FCM | 1/10-1/50 |
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GIF Antibody (Center) (Cat. #P31192) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GIF antibody detected the GIF protein (arrow). |
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Confocal immunofluorescent analysis of GIF Antibody (Center) (Cat#P31192) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
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GIF Antibody (Center) (Cat. #P31192) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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GIF Antibody (Center) (Cat. #P31192)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GIF Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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