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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to GNG3

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别名:Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3, GNG3, GNGT3
应用:WB,FCM
反应种属:Human, Mouse
规格:50μl/100μl

Description
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction.
Specification
Aliases Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3, GNG3, GNGT3
Entrez GeneID 2785
Swissprot P63215
WB Predicted band size 8.3kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This GNG3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 39-69 amino acids from human GNG3.
Application
WB 1/2000
FCM 1/25

All lanes : Anti-GNG3 Antibody (C-Term) at 1:2000 dilution
Lane 1: human fetal brain lysate
Lane 2: mouse brain lysate
Lane 3: human brain lysate
Lane 4: mouse cerebellum lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 8 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing U-87 MG cells stained with P34177(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34177, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。