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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to GPRC6A

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别名:G-protein coupled receptor family C group 6 member A; hGPRC6A; G-protein coupled receptor GPCR33; hGPCR33
应用:WB,ICC
反应种属:Human,Mouse,Rat
规格:50μl/100μl

Specification
Aliases G-protein coupled receptor family C group 6 member A; hGPRC6A; G-protein coupled receptor GPCR33; hGPCR33
Entrez GeneID 222545;
Swissprot Q5T6X5
WB Predicted band size 120kDa
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPRC6A.
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Application
WB 1/500-1/1000
ICC 1/100-1/300

Western blot analysis of ZIP1 expression in H1792, LO2, HCT116, PC12, AML12 whole cell lysates.
Immunofluorescent analysis of GPRC6A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。