中文 
别名:Gasdermin-C, Melanoma-derived leucine zipper-containing extranuclear factor, GSDMC, MLZE应用:WB,FCM
反应种属:Human
规格：50μl/100μl
| Specification | |
|---|---|
| Aliases | Gasdermin-C, Melanoma-derived leucine zipper-containing extranuclear factor, GSDMC, MLZE |
| Entrez GeneID | 56169 |
| Swissprot | Q9BYG8 |
| WB Predicted band size | 57.7kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GSDMC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human GSDMC. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Application | |
|---|---|
| WB | 1/1000 |
| FCM | 1/10-1/50 |
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All lanes : Anti-GSDMC Antibody (Center) at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: SK-MEL-2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Overlay histogram showing U-2OS cells stained with P34311 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34311, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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