Rabbit Polyclonal Antibody to GSTM5 (N-term)

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别名:Glutathione S-transferase Mu 5, GST class-mu 5, GSTM5-5, GSTM5
应用:WB,IHC,FCM
反应种属:Human
规格：50μl/100μl

Description
Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual’s susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Diversification of these genes has occurred in regions encoding substrate-binding domains, as well as in tissue expression patterns, to accommodate an increasing number of foreign compounds.

Description

Cytosolic and membrane-bound forms of glutathione
S-transferase are encoded by two distinct supergene families. At
present, eight distinct classes of the soluble cytoplasmic
mammalian glutathione S-transferases have been identified: alpha,
kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a
glutathione S-transferase that belongs to the mu class. The mu
class of enzymes functions in the detoxification of electrophilic
compounds, including carcinogens, therapeutic drugs, environmental
toxins and products of oxidative stress, by conjugation with
glutathione. The genes encoding the mu class of enzymes are
organized in a gene cluster on chromosome 1p13.3 and are known to
be highly polymorphic. These genetic variations can change an
individual’s susceptibility to carcinogens and toxins as well as
affect the toxicity and efficacy of certain drugs. Diversification
of these genes has occurred in regions encoding substrate-binding
domains, as well as in tissue expression patterns, to accommodate
an increasing number of foreign compounds.

Specification
Aliases	Glutathione S-transferase Mu 5, GST class-mu 5, GSTM5-5, GSTM5
Entrez GeneID	2949
Swissprot	P46439
WB Predicted band size	25.7kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This GSTM5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-43 amino acids from the N-terminal region of human GSTM5.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	Western blot analysis of lysate from human brain tissue lysate, using GSTM5 Antibody (N-term)(Cat. #P31139). P31139 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
	GSTM5 Antibody (N-term) (Cat. #P31139) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	GSTM5 Antibody (N-term) (Cat. #P31139)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GSTM5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.