别名:Glycogenin-1, GN-1, GN1, 2.4.1.186, GYG1, GYG应用:WB,FCM
反应种属:Human, Mouse, Rat
规格:50μl/100μl
Description |
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Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase. |
Specification | |
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Aliases | Glycogenin-1, GN-1, GN1, 2.4.1.186, GYG1, GYG |
Entrez GeneID | 2992 |
Swissprot | P46976 |
WB Predicted band size | 39.4kDa |
Host/Isotype | Rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | This GYG1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 314-347 amino acids from human GYG1. |
Application | |
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WB | 1/2000 |
FCM | 1/25 |
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All lanes : Anti-GYG1 Antibody (C-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse heart lysate Lane 4: Mouse skeletal muscle lysate Lane 5: Rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Overlay histogram showing HepG2 cells stained with P34462(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34462, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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