Rabbit Polyclonal Antibody to H Cadherin (CDH13) (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:Cadherin-13, Heart cadherin, H-cadherin, P105, Truncated cadherin, T-cad, T-cadherin, CDH13, CDHH
应用:WB,IHC,FCM
反应种属:Human, Mouse
规格：50μl/100μl

Description
CDH13 is a member of the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region but, unlike the typical cadherin superfamily member, lacks the highly conserved cytoplasmic region. This particular cadherin is a putative mediator of cell-cell interaction in the heart and may act as a negative regulator of neural cell growth.

Description

CDH13 is a member of the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region but, unlike the typical cadherin superfamily member, lacks the highly conserved cytoplasmic region. This particular cadherin is a putative mediator of cell-cell interaction in the heart and may act as a negative regulator of neural cell growth.

Specification
Aliases	Cadherin-13, Heart cadherin, H-cadherin, P105, Truncated cadherin, T-cad, T-cadherin, CDH13, CDHH
Entrez GeneID	1012
Swissprot	P55290
WB Predicted band size	78.3kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	This H Cadherin (CDH13) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-174 amino acids from the N-terminal region of human H Cadherin (CDH13).
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	Western blot analysis of lysate from human brain tissue lysate, using CDH13 Antibody (N-term)(Cat. #P30243). P30243 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
	Flow cytometric analysis of HepG2 cells using H Cadherin (CDH13) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human brain tissue with H Cadherin (CDH13) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.