Rabbit Polyclonal Antibody to HDAC2

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别名:HDAC2; Histone deacetylase 2; HD2
应用:WB,IHC,ICC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA.

Description

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA.

Specification
Aliases	HDAC2; Histone deacetylase 2; HD2
Entrez GeneID	3066
Swissprot	Q92769
WB Predicted band size	Calculated MW: 55 kDa; Observed MW: 60 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Peptide sequence around aa. 392~396 (E-D-S-G-D) derived from Human HDAC2.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200

	Western blot analysis of HDAC2 in 293, 3T3 and COS7 lysates using HDAC2 (Ab394) antibody.
	Immunofluorescence analysis of HDAC2 in Hela using HDAC2 (Ab394) antibody.
	Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using HDAC2 (Ab394) antibody (left) or the same antibody preincubated with blocking peptide (right).High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.