Rabbit Polyclonal Antibody to HIF1 alpha

包装: 1G|100G|500G|1KG|100KG

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别名:HIF1A; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS doma
应用:WB,IHC,ICC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1.

Description

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1.

Specification
Aliases	HIF1A; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS doma
Entrez GeneID	3091
Swissprot	Q16665
WB Predicted band size	Calculated MW: 93 kDa; Observed MW: 92-130 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	The antiserum was produced against synthesized peptide derived from human HIF-1alpha. AA range:328-377
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200
ELISA	1/10000

	Western blot analysis of HIF1 alpha in LOVO lysates using HIF1 alpha antibody.
	Immunohistochemistry analysis of paraffin-embedded mouse colon tissue using HIF1 alpha antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
	Immunohistochemistry analysis of paraffin-embedded rat lung tissue using HIF1 alpha antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.