Rabbit Polyclonal Antibody to HIF1AN

包装: 1G|100G|500G|1KG|100KG

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别名:FIH1
应用:WB,IHC
反应种属:Human, Mouse
规格：50μl/100μl

Description
Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha. This hydroxylation event blocks the association of HIF-1alpha with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm.

Description

Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha. This hydroxylation event blocks the association of HIF-1alpha with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm.

Specification
Aliases	FIH1
Swissprot	Q9NWT6
WB Predicted band size	40 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	Synthetic peptide of human HIF1AN
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/500-1/2000
IHC	1/50-1/300
ELISA	1/2000-1/10000

	Gel: 8%SDS-PAGE, Lysate: 60 μg, Lane 1-2: Mouse brain tissue, Mouse liver tissue, Primary antibody: P05210(HIF1AN Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
	The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P05210(HIF1AN Antibody) at dilution 1/40. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human brain tissue using P05210(HIF1AN Antibody) at dilution 1/40. (Original magnification: ×200)