Rabbit Polyclonal Antibody to HINT1

包装: 1G|100G|500G|1KG|100KG

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别名:adenosine 5′-monophosphoramidase; EC 3.-.-.-; histidine triad nucleotide-binding 1; PKCI-1; PKCI1
应用:WB,ICC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Hydrolyzes purine nucleotide phosphoramidates with a single phosphate group, including adenosine 5’monophosphoramidate (AMP-NH2), adenosine 5’monophosphomorpholidate (AMP-morpholidate) and guanosine 5’monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Can also convert adenosine 5′-O-phosphorothioate and guanosine 5′-O-phosphorothioate to the corresponding nucleoside 5′-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex.Brzoska P.M., Genomics 36:151-156(1996).Brzoska P.M., Proc. Natl. Acad. Sci. U.S.A. 92:7824-7828(1995).Ota T., Nat. Genet. 36:40-45(2004).

Description

Hydrolyzes purine nucleotide phosphoramidates with a single phosphate group, including adenosine 5’monophosphoramidate (AMP-NH2), adenosine 5’monophosphomorpholidate (AMP-morpholidate) and guanosine 5’monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Can also convert adenosine 5′-O-phosphorothioate and guanosine 5′-O-phosphorothioate to the corresponding nucleoside 5′-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex.Brzoska P.M., Genomics 36:151-156(1996).Brzoska P.M., Proc. Natl. Acad. Sci. U.S.A. 92:7824-7828(1995).Ota T., Nat. Genet. 36:40-45(2004).

Specification
Aliases	adenosine 5′-monophosphoramidase; EC 3.-.-.-; histidine triad nucleotide-binding 1; PKCI-1; PKCI1
Entrez GeneID	3094;
Swissprot	P49773
WB Predicted band size	28kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from internal of human HINT1.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
ICC	1/100-1/500

	Western blot analysis of extracts from Jurkat cells, using HINT1 antibody P41953.
	Immunofluorescence analysis of HeLa cells, using HINT1 antibody P41953.