Rabbit Polyclonal Antibody to HIST1H2AB (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:Histone H2A type 1-B/E, Histone H2A2, Histone H2A/a, Histone H2A/m, HIST1H2AB, H2AFM
应用:WB,IHC,FCM
反应种属:Human
规格：50μl/100μl

Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Description

Histones are basic nuclear proteins that are responsible
for the nucleosome structure of the chromosomal fiber in
eukaryotes. This structure consists of approximately 146 bp of DNA
wrapped around a nucleosome, an octamer composed of pairs of each
of the four core histones (H2A, H2B, H3, and H4). The chromatin
fiber is further compacted through the interaction of a linker
histone, H1, with the DNA between the nucleosomes to form higher
order chromatin structures. This gene is intronless and encodes a
member of the histone H2A family. Transcripts from this gene lack
polyA tails; instead, they contain a palindromic termination
element. This gene is found in the large histone gene cluster on
chromosome 6p22-p21.3.

Specification
Aliases	Histone H2A type 1-B/E, Histone H2A2, Histone H2A/a, Histone H2A/m, HIST1H2AB, H2AFM
Entrez GeneID	3012;8335
Swissprot	P04908
WB Predicted band size	14.1kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This HIST1H2AB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human HIST1H2AB.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	HIST1H2AB Antibody (N-term) (Cat. #P31212) western blot analysis in K562,CEM,MDA-MB453,NCI-H460,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HIST1H2AB antibody detected the HIST1H2AB protein (arrow).
	HIST1H2AB Antibody (N-term) (Cat. #P31212) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
	HIST1H2AB Antibody (N-term) (Cat. #P31212)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIST1H2AB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.