Rabbit Polyclonal Antibody to Histone H2AX

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别名:H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X
应用:WB,ICC
反应种属:Human
规格：50μl/100μl

Description
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.The MGC Project Team; Genome Res. 14:2121-2127(2004).Rogakou E.P., J. Biol. Chem. 273:5858-5868(1998).Rogakou E.P., J. Biol. Chem. 275:9390-9395(2000).

Description

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.The MGC Project Team; Genome Res. 14:2121-2127(2004).Rogakou E.P., J. Biol. Chem. 273:5858-5868(1998).Rogakou E.P., J. Biol. Chem. 275:9390-9395(2000).

Specification
Aliases	H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X
Entrez GeneID	3014;
Swissprot	P16104
WB Predicted band size	15kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized peptide derived from internal of human Histone H2AX.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
ICC	1/100-1/500

	Western blot analysis of extracts from HT-29 cells, using Histone H2AX antibody P41911.
	Immunofluorescence analysis of COS7 cells, using Histone H2AX antibody P41911.