Rabbit Polyclonal Antibody to HLA-DQA1 (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:HLA class II histocompatibility antigen, DQ alpha 1 chain, DC-1 alpha chain, DC-alpha, HLA-DCA, MHC class II DQA1, HLA-DQA1
应用:WB,FCM
反应种属:Human
规格：50μl/100μl

Description
HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.

Description

HLA-DQA1 belongs to the HLA class II alpha chain
paralogues. The class II molecule is a heterodimer consisting of an
alpha (DQA) and a beta chain (DQB), both anchored in the membrane.
It plays a central role in the immune system by presenting peptides
derived from extracellular proteins. Class II molecules are
expressed in antigen presenting cells (APC: B Lymphocytes,
dendritic cells, macrophages). The alpha chain is approximately
33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader
peptide, exons 2 and 3 encode the two extracellular domains, and
exon 4 encodes the transmembrane domain and the cytoplasmic tail.
Within the DQ molecule both the alpha chain and the beta chain
contain the polymorphisms specifying the peptide binding
specificities, resulting in up to four different molecules. Typing
for these polymorphisms is routinely done for bone marrow
transplantation.

Specification
Aliases	HLA class II histocompatibility antigen, DQ alpha 1 chain, DC-1 alpha chain, DC-alpha, HLA-DCA, MHC class II DQA1, HLA-DQA1
Entrez GeneID	3117
Swissprot	P01909
WB Predicted band size	27.8kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This HLA-DQA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-66 amino acids from the N-terminal region of human HLA-DQA1.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
FCM	1/10-1/50

	Anti-HLA-DQA1 Antibody (N-term) at 1:1000 dilution + Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	HLA-DQA1 Antibody (N-term) (Cat. #P33527) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the HLA-DQA1 antibody detected the HLA-DQA1 protein (arrow).
	Western blot analysis of HLA-DQA1 (arrow) using rabbit polyclonal HLA-DQA1 Antibody (N-term) (Cat. #P33527). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-DQA1 gene.
	HLA-DQA1 Antibody (N-term) (Cat. #P33527) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.