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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to HLA-DRA (N-term)

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别名:HLA class II histocompatibility antigen, DR alpha chain, MHC class II antigen DRA, HLA-DRA, HLA-DRA1
应用:WB,IHC,FCM
反应种属:Human
规格:50μl/100μl

Description
HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages).
Specification
Aliases HLA class II histocompatibility antigen, DR alpha chain, MHC class II antigen DRA, HLA-DRA, HLA-DRA1
Entrez GeneID 3122
Swissprot P01903
WB Predicted band size 28.6kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This HLA-DRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-75 amino acids from the N-terminal region of human HLA-DRA.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/500-1/1000
IHC 1/100-1/500
FCM 1/10-1/50

All lanes : Anti-NSUN7 Antibody (C-term) at 1:500 dilution
Lane 1: Daudi whole cell lysate
Lane 2: Raji whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution.

Observed band size : 32kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of HLA-DRA Antibody (N-term) (Cat. #P34942) in Ramos cell line lysates (35ug/lane). HLA-DRA (arrow) was detected using the purified Pab.
HLA-DRA Antibody (N-term) (Cat. #P34942) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
HLA-DRA Antibody (N-term) (Cat. #P34942) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HLA-DRA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。