别名:HLA class II histocompatibility antigen, DR alpha chain, MHC class II antigen DRA, HLA-DRA, HLA-DRA1应用:WB,IHC,FCM
反应种属:Human
规格:50μl/100μl
Description |
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HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). |
Specification | |
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Aliases | HLA class II histocompatibility antigen, DR alpha chain, MHC class II antigen DRA, HLA-DRA, HLA-DRA1 |
Entrez GeneID | 3122 |
Swissprot | P01903 |
WB Predicted band size | 28.6kDa |
Host/Isotype | Rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This HLA-DRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-75 amino acids from the N-terminal region of human HLA-DRA. |
Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Application | |
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WB | 1/500-1/1000 |
IHC | 1/100-1/500 |
FCM | 1/10-1/50 |
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All lanes : Anti-NSUN7 Antibody (C-term) at 1:500 dilution Lane 1: Daudi whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 32kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Western blot analysis of HLA-DRA Antibody (N-term) (Cat. #P34942) in Ramos cell line lysates (35ug/lane). HLA-DRA (arrow) was detected using the purified Pab. |
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HLA-DRA Antibody (N-term) (Cat. #P34942) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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HLA-DRA Antibody (N-term) (Cat. #P34942) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HLA-DRA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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