Rabbit Polyclonal Antibody to HMGB1

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别名:HMG1; HMG3; SBP-1
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NF˚B family, ATF-2 and c-Jun to activate transcription. Other studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein. Human HMG-1 and HMG-2 both contain two DNA-binding domains, termed HMG boxes. HMG proteins bind single-stranded DNA but induce conformational changes in double-stranded DNA alone.

Description

High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NF˚B family, ATF-2 and c-Jun to activate transcription. Other studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein. Human HMG-1 and HMG-2 both contain two DNA-binding domains, termed HMG boxes. HMG proteins bind single-stranded DNA but induce conformational changes in double-stranded DNA alone.

Specification
Aliases	HMG1; HMG3; SBP-1
Swissprot	P09429
WB Predicted band size	25 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide of human HMGB1
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/500-1/2000
IHC	1/50-1/200
ELISA	1/2000-1/5000

	Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-5: Human hepatocellular carcinoma tissue, Hela cells, Jurkat cells, 293T cells, human breast infiltrative duct tissue, Primary antibody: P10952(HMGB1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
	The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P10952(HMGB1 Antibody) at dilution 1/30. (Original magnification: ×200)