Rabbit Polyclonal Antibody to HNF4A

包装: 1G|100G|500G|1KG|100KG

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别名:Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, TCF-14, Transcription factor HNF-4, HNF4A, HNF4, NR2A1, TCF14
应用:WB,IHC,ICC,FCM
反应种属:Human, Rat
规格：50μl/100μl

Description
HNF4A is a nuclear transcription factor which binds DNA as a homodimer. This protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This protein may play a role in development of the liver, kidney, and intestines. Mutations in HNF4A gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I.

Description

HNF4A is a nuclear transcription factor which binds DNA as a homodimer. This protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This protein may play a role in development of the liver, kidney, and intestines. Mutations in HNF4A gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I.

Specification
Aliases	Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, TCF-14, Transcription factor HNF-4, HNF4A, HNF4, NR2A1, TCF14
Entrez GeneID	3172
Swissprot	P41235
WB Predicted band size	52.8kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Rat
Immunogen	This HNF4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-312 amino acids from the Central region of human HNF4A.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Application
WB	1/1000
IHC	1/100-1/500
ICC	1/10-1/50
FCM	1/10-1/50

	HNF4A Antibody (Center) (Cat. #P30337) western blot analysis in 293,HepG2 cell line and rat liver tissue lysates (35ug/lane).This demonstrates the HNF4A antibody detected the HNF4A protein (arrow).
	Confocal immunofluorescent analysis of HNF4A Antibody (Center)(Cat#P30337) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
	HNF4A Antibody (Center) (Cat. #P30337) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	HNF4A Antibody (Center) (P30337)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HNF4A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.