Rabbit Polyclonal Antibody to HOXA10

包装: 1G|100G|500G|1KG|100KG

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别名:Homeobox protein Hox-A10, Homeobox protein Hox-18, Homeobox protein Hox-1H, PL, HOXA10, HOX1H
应用:WB,FCM
反应种属:Human
规格：50μl/100μl

Description
In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor that may regulate gene expression, morphogenesis, and differentiation. More specifically, it may function in fertility, embryo viability, and regulation of hematopoietic lineage commitment. Alternatively spliced transcript variants encoding different isoforms have been described.

Description

In vertebrates, the genes encoding the class of
transcription factors called homeobox genes are found in clusters
named A, B, C, and D on four separate chromosomes. Expression of
these proteins is spatially and temporally regulated during
embryonic development. This gene is part of the A cluster on
chromosome 7 and encodes a DNA-binding transcription factor that
may regulate gene expression, morphogenesis, and differentiation.
More specifically, it may function in fertility, embryo viability,
and regulation of hematopoietic lineage commitment. Alternatively
spliced transcript variants encoding different isoforms have been
described.

Specification
Aliases	Homeobox protein Hox-A10, Homeobox protein Hox-18, Homeobox protein Hox-1H, PL, HOXA10, HOX1H
Entrez GeneID	3206
Swissprot	P31260
WB Predicted band size	42.4kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This HOXA10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-271 amino acids from the Central region of human HOXA10.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
FCM	1/10-1/50

	Western blot analysis of lysate from T47D cell line, using HOXA10 Antibody (Center)(Cat. #P32783). P32783 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
	HOXA10 Antibody (Center) (Cat. #P32783) western blot analysis in WiDr,HepG2 cell line lysates (35ug/lane).This demonstrates the HOXA10 antibody detected the HOXA10 protein (arrow).
	Confocal immunofluorescent analysis of HOXA10 Antibody (Center) (Cat#P32783) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).