Rabbit Polyclonal Antibody to HP

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别名:Haptoglobin, Zonulin, Haptoglobin alpha chain, Haptoglobin beta chain, HP
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn’s disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson’s disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene.

Description

This gene encodes a preproprotein, which is processed to
yield both alpha and beta chains, which subsequently combine as a
tetramer to produce haptoglobin. Haptoglobin functions to bind free
plasma hemoglobin, which allows degradative enzymes to gain access
to the hemoglobin, while at the same time preventing loss of iron
through the kidneys and protecting the kidneys from damage by
hemoglobin. Mutations in this gene and/or its regulatory regions
cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also
been linked to diabetic nephropathy, the incidence of coronary
artery disease in type 1 diabetes, Crohn’s disease, inflammatory
disease behavior, primary sclerosing cholangitis, susceptibility to
idiopathic Parkinson’s disease, and a reduced incidence of
Plasmodium falciparum malaria. A similar duplicated gene is located
next to this gene on chromosome 16. Multiple transcript variants
encoding different isoforms have been found for this gene.

Specification
Aliases	Haptoglobin, Zonulin, Haptoglobin alpha chain, Haptoglobin beta chain, HP
Entrez GeneID	3240
Swissprot	P00738
WB Predicted band size	45.2kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This HP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-322 amino acids from the Central region of human HP.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/500

	Anti-HP Antibody (Center) at 1:2000 dilution + Human plasma lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	Immunohistochemical analysis of paraffin-embedded Human hepatocarcinoma tissue using P34735 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
	Immunohistochemical analysis of paraffin-embedded Human hepatocarcinoma tissue using P34735 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
	Immunohistochemical analysis of paraffin-embedded Human colon tissue using P34735 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
	Immunohistochemical analysis of paraffin-embedded Human hepatocarcinoma tissue using P34735 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.