库存-现货
瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to HPX

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别名:Hemopexin, Beta-1B-glycoprotein, HPX
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
HPX is a plasma glycoprotein that binds heme with high affinity. The protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress.
Specification
Aliases Hemopexin, Beta-1B-glycoprotein, HPX
Entrez GeneID 3263
Swissprot P02790
WB Predicted band size 51.7kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This HPX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-227 amino acids from the Central region of human HPX.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/2000
IHC 1/500

All lanes : Anti-HPX Antibody (Center) at 1:2000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: Human plasma lysate
Lane 3: Mouse plasma lysate
Lane 4: Rat plasma lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 52 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded Human liver tissue using P34731 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。