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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to HSPA1A/HSPA1B (Y41)

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别名:Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP701/HSP702, HSPA1A, HSPA1, HSX70
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
HSPA1A is a member of the heat shock protein 70 family. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1.
Specification
Aliases Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP701/HSP702, HSPA1A, HSPA1, HSX70
Swissprot P08107
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This HSPA1A/HSPA1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-48 amino acids from human HSPA1A/HSPA1B.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/4000
IHC 1/100

All lanes : Anti-HSPA1A(Y41) Antibody at 1:4000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: 293 whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: C2C12 whole cell lysate
Lane 6: NIH/3T3 whole cell lysate
Lane 7: C6 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 70 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of P34781 on paraffin-embedded Human colon tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of P34781 on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。