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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to HTR1E

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别名:5-hydroxytryptamine receptor 1E, 5-HT-1E, 5-HT1E, S31, Serotonin receptor 1E, HTR1E
应用:WB,IHC
反应种属:Human
规格:50μl/100μl

Description
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.
Specification
Aliases 5-hydroxytryptamine receptor 1E, 5-HT-1E, 5-HT1E, S31, Serotonin receptor 1E, HTR1E
Entrez GeneID 3354
Swissprot P28566
WB Predicted band size 41.7kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This HTR1E antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 223-258 amino acids from human HTR1E.
Application
WB 1/2000
IHC 1/100-1/500

All lanes : Anti-HTR1E Antibody (C-Term) at 1:2000 dilution
Lane 1: DU145 whole cell lysate
Lane 2: human brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 42 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

P33356 staining HTR1E in human kidney tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。