别名:Isoleucine–tRNA ligase, mitochondrial, Isoleucyl-tRNA synthetase, IleRS, IARS2应用:WB,IHC
反应种属:Human
规格:50μl/100μl
Description |
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IARS2 belongs to the class-I aminoacyl-tRNA synthetase family. Members of class I have two highly conserved sequence motifs. They aminoacylate at the 2′-OH of an adenosine nucleotide, and are usually monomeric or dimeric (one or two subunits, respectively). Both classes of aminoacyl-tRNA synthetases are multidomain proteins. The catalytic domains of all the aaRSs of a given class are found to be homologous to one another, while class I and class II aaRSs are unrelated to one another. The class I aaRSs have the ubiquitous Rossmann fold and have the antiparallel beta-strands architecture while the class II aaRSs have a unique fold made up of antiparallel beta-strands. |
Specification | |
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Aliases | Isoleucine–tRNA ligase, mitochondrial, Isoleucyl-tRNA synthetase, IleRS, IARS2 |
Entrez GeneID | 55699 |
Swissprot | Q9NSE4 |
WB Predicted band size | 113.8kDa |
Host/Isotype | Rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This IARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 600-628 amino acids from the Central region of human IARS2. |
Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Application | |
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WB | 1/1000 |
IHC | 1/100-1/500 |
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All lanes : Anti-IARS2 Antibody (Center) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 114 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with IARS2 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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