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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to IARS2

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别名:Isoleucine–tRNA ligase, mitochondrial, Isoleucyl-tRNA synthetase, IleRS, IARS2
应用:WB,IHC
反应种属:Human
规格:50μl/100μl

Description
IARS2 belongs to the class-I aminoacyl-tRNA synthetase family. Members of class I have two highly conserved sequence motifs. They aminoacylate at the 2′-OH of an adenosine nucleotide, and are usually monomeric or dimeric (one or two subunits, respectively). Both classes of aminoacyl-tRNA synthetases are multidomain proteins. The catalytic domains of all the aaRSs of a given class are found to be homologous to one another, while class I and class II aaRSs are unrelated to one another. The class I aaRSs have the ubiquitous Rossmann fold and have the antiparallel beta-strands architecture while the class II aaRSs have a unique fold made up of antiparallel beta-strands.
Specification
Aliases Isoleucine–tRNA ligase, mitochondrial, Isoleucyl-tRNA synthetase, IleRS, IARS2
Entrez GeneID 55699
Swissprot Q9NSE4
WB Predicted band size 113.8kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This IARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 600-628 amino acids from the Central region of human IARS2.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/1000
IHC 1/100-1/500

All lanes : Anti-IARS2 Antibody (Center) at 1:1000 dilution
Lane 1: K562 whole cell lysate
Lane 2: 293 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 114 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with IARS2 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。