库存-现货
瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to IFITM5

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别名:Interferon-induced transmembrane protein 5, Bone-restricted interferon-induced transmembrane protein-like protein, BRIL, Dispanin subfamily A member 1, DSPA1, IFITM5
应用:WB,FCM
反应种属:Human, Mouse
规格:50μl/100μl

Description
Plays a role in bone mineralization (By similarity).
Specification
Aliases Interferon-induced transmembrane protein 5, Bone-restricted interferon-induced transmembrane protein-like protein, BRIL, Dispanin subfamily A member 1, DSPA1, IFITM5
Entrez GeneID 387733
Swissprot A6NNB3
WB Predicted band size 14.4kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This IFITM5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-83 amino acids of human IFITM5.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/2000
FCM 1/25

All lanes : Anti-IFITM5 Antibody (Center) at 1:4000 dilution
Lane 1: T47D whole cell lysate
Lane 2: PC-3 whole cell lysate
Lane 3: SW480 whole cell lysate
Lane 4: HT-29 whole cell lysate
Lane 5: PANC-1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 14 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing A431 cells stained with P34544(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。