Rabbit Polyclonal Antibody to IKK-γ (Ab-85)

包装: 1G|100G|500G|1KG|100KG

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别名:FIP-3; FIP3; I-kappa-B kinase gamma; IKBKG; IKK-gamma
应用:WB,ICC
反应种属:Human
规格：50μl/100μl

Description
Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either ‘Lys-63’-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires ‘Lys-27’-linked polyubiquitination.Li Y., Proc. Natl. Acad. Sci. U.S.A. 96:1042-1047(1999).Jin D.-Y., J. Biomed. Sci. 6:115-120(1999).Rothwarf D.M., Nature 395:297-300(1998).

Description

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either ‘Lys-63’-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires ‘Lys-27’-linked polyubiquitination.Li Y., Proc. Natl. Acad. Sci. U.S.A. 96:1042-1047(1999).Jin D.-Y., J. Biomed. Sci. 6:115-120(1999).Rothwarf D.M., Nature 395:297-300(1998).

Specification
Aliases	FIP-3; FIP3; I-kappa-B kinase gamma; IKBKG; IKK-gamma
Entrez GeneID	8517;
Swissprot	Q9Y6K9
WB Predicted band size	43kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized non-phosphopeptide derived from human IKK-γ around the phosphorylation site of serine 85 (Q-A-S(p)-Q-R).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
ICC	1/100-1/500

	Western blot analysis of extracts from HepG2 cells, treated with Anisomycin (0.5uM, 5hours), using IKK-γ(Ab-85) antibody P41683.
	Immunofluorescence analysis of HeLa cells, using IKK-γ(Ab-85) antibody P41683.