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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to IL1RL2

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别名:Interleukin-1 receptor-like 2, IL-36 receptor, IL-36R, Interleukin-1 receptor-related protein 2, IL-1Rrp2, IL1R-rp2, IL1RL2, IL1RRP2
应用:WB,FCM
反应种属:Human
规格:50μl/100μl

Description
The protein encoded by this gene is a member of the
interleukin 1 receptor family. An experiment with transient gene
expression demonstrated that this receptor was incapable of binding
to interleukin 1 alpha and interleukin 1 beta with high affinity.
This gene and four other interleukin 1 receptor family genes,
including interleukin 1 receptor, type I (IL1R1), interleukin 1
receptor, type II (IL1R2), interleukin 1 receptor-like 1 (IL1RL1),
and interleukin 18 receptor 1 (IL18R1), form a cytokine receptor
gene cluster in a region mapped to chromosome 2q12. [provided by
RefSeq].
Specification
Aliases Interleukin-1 receptor-like 2, IL-36 receptor, IL-36R, Interleukin-1 receptor-related protein 2, IL-1Rrp2, IL1R-rp2, IL1RL2, IL1RRP2
Entrez GeneID 8808
Swissprot Q9HB29
WB Predicted band size 65.4kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This IL1RL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the Central region of human IL1RL2.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
FCM 1/25

All lanes : Anti-IL1RL2 Antibody (Center) at 1:8000 dilution
Lane 1: HL-60 whole cell lysate
Lane 2: U-87 MG whole cell lysate
Lane 3: K562 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 65 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing K562 cells stained with P35095 (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P35095, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。