Rabbit Polyclonal Antibody to JAK3 (Ab-785)

包装: 1G|100G|500G|1KG|100KG

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别名:EC 2.7.10.2; JAK-3; JAK3; Janus kinase 3; L-JAK
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.Kawamura M., Proc. Natl. Acad. Sci. U.S.A. 91:6374-6378(1994).Lai K.S., J. Biol. Chem. 270:25028-25036(1995).Grimwood J., Nature 428:529-535(2004).

Description

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.Kawamura M., Proc. Natl. Acad. Sci. U.S.A. 91:6374-6378(1994).Lai K.S., J. Biol. Chem. 270:25028-25036(1995).Grimwood J., Nature 428:529-535(2004).

Specification
Aliases	EC 2.7.10.2; JAK-3; JAK3; Janus kinase 3; L-JAK
Entrez GeneID	3718;
Swissprot	P52333
WB Predicted band size	125kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized non-phosphopeptide derived from human JAK3 around the phosphorylation site of tyrosine 785 (S-D-Y(p)-E-L).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from Jurkat cells, using JAK3 (Ab-785) antibody P41674.
	Immunohistochemistry analysis of paraffin-embedded human brain tissue using JAK3 (Ab-785) antiobdy P41674.