中文 
别名:Potassium voltage-gated channel subfamily H member 1, Ether-a-go-go potassium channel 1, EAG channel 1, h-eag, hEAG1, Voltage-gated potassium channel subunit Kv10.1, KCNH1, EAG, EAG1应用:WB,ICC,FCM
反应种属:Human, Mouse, Rat
规格：50μl/100μl
| Description |
|---|
| Pore-forming (alpha) subunit of voltage-gated non- inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts. |
| Specification | |
|---|---|
| Aliases | Potassium voltage-gated channel subfamily H member 1, Ether-a-go-go potassium channel 1, EAG channel 1, h-eag, hEAG1, Voltage-gated potassium channel subunit Kv10.1, KCNH1, EAG, EAG1 |
| Entrez GeneID | 3756 |
| Swissprot | O95259 |
| WB Predicted band size | 111.4kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This KCNH1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 787-820 amino acids from the human region of human KCNH1. |
| Application | |
|---|---|
| WB | 1/2000 |
| ICC | 1/25 |
| FCM | 1/25 |
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All lanes : Anti-KCNH1 Antibody (C-Term) at 1:2000 dilution Lane 1: COLO 205 whole cell lysate Lane 2: Human brain lysate Lane 3: Hela whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 111 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela cells labeling KCNH1 with P34534 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Nucleus and Cytoplasm staining on Hela cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin(red). The nuclear counter stain is DAPI (blue). |
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Overlay histogram showing Hela cells stained with P34534(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34534, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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